Forgot one project last night: analysis of mRNA levels in multiple mouse tissues of our genes, as well as determination of an appropriate control to use. Hey! Any suggestions? I’m currently trying 18S rRNA, beta-actin, and TBP. I’ve used the 18S and beta-actin before but the level of expression of our genes is so low that I’m at least 10 Ct values away. I just read about TBP as a control, so I’ve got primers to try that. We’re looking at brain, liver, heart, spleen, and lung tissue right now, but will want to include any tissue we can cleanly isolate.